Composite

Part:BBa_K2824006:Design

Designed by: Jiang Qiaochu   Group: iGEM18_NEU_China_B   (2018-10-05)


T7-lldPRD operon promoter-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 421
    Illegal BsaI.rc site found at 1080


Design Notes

When constructing part, we should remove the intermediate restriction site. We can intercept and connect separately.


Source

LLDPRD gene was extracted from the K12 genome.T7 promoter was copied from the PCDFDuet plasmid . GFP gene was a commercial components which is a green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP .

References